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nsii integrant cell line  (New England Biolabs)


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    New England Biolabs nsii integrant cell line
    Nsii Integrant Cell Line, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsii+integrant+cell+line/NsiI-HF/pmc11444041-79-25-61
    Average 95 stars, based on 82 article reviews
    nsii integrant cell line - by Bioz Stars, 2026-09
    95/100 stars

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    Mutagenesis:

    Article Title: SMAD4 mutations causing Myhre syndrome are under positive selection in the male germline.
    Article Snippet: .. Individual biological samples (sperm or blood) were processed in triplicate: each technical replicate comprising 10 mg of gDNA spiked with 0.6 ng DNA from the NsiI-Integrant cell line (corresponding to a final dilution of 3 3 10 5 or 100 mutant haploid genomes) was digested in a 100 mL volume reaction containing 4 The American Journal of Human Genetics 111, 1–17, September 5, 20 U NsiI-HF and 20 U PstI-HF (both from New England BioLabs) for 2 h at 37 C, after which another 20 U of each enzyme was added and incubated for a further 2 h. NsiI mutant sequences generate a 1,332 bp PstI fragment, while the wild-type sequences are cleaved by NsiI into 2 fragments (488 and 844 bp). .. Each digest was loaded on a 1% agarose/TAE gel and flanked by a l DNA/EcoRI ladder (Fermentas).

    Article Title: SMAD4 mutations causing Myhre syndrome are under positive selection in the male germline
    Article Snippet: .. Individual biological samples (sperm or blood) were processed in triplicate: each technical replicate comprising 10 μg of gDNA spiked with 0.6 ng DNA from the NsiI-Integrant cell line (corresponding to a final dilution of 3 × 10 −5 or ∼100 mutant haploid genomes) was digested in a 100 μL volume reaction containing 20 U NsiI-HF and 20 U PstI-HF (both from New England BioLabs) for 2 h at 37°C, after which another 20 U of each enzyme was added and incubated for a further 2 h. NsiI mutant sequences generate a 1,332 bp PstI fragment, while the wild-type sequences are cleaved by NsiI into 2 fragments (488 and 844 bp). .. Each digest was loaded on a 1% agarose/TAE gel and flanked by a λ DNA/EcoRI ladder (Fermentas).

    Incubation:

    Article Title: SMAD4 mutations causing Myhre syndrome are under positive selection in the male germline.
    Article Snippet: .. Individual biological samples (sperm or blood) were processed in triplicate: each technical replicate comprising 10 mg of gDNA spiked with 0.6 ng DNA from the NsiI-Integrant cell line (corresponding to a final dilution of 3 3 10 5 or 100 mutant haploid genomes) was digested in a 100 mL volume reaction containing 4 The American Journal of Human Genetics 111, 1–17, September 5, 20 U NsiI-HF and 20 U PstI-HF (both from New England BioLabs) for 2 h at 37 C, after which another 20 U of each enzyme was added and incubated for a further 2 h. NsiI mutant sequences generate a 1,332 bp PstI fragment, while the wild-type sequences are cleaved by NsiI into 2 fragments (488 and 844 bp). .. Each digest was loaded on a 1% agarose/TAE gel and flanked by a l DNA/EcoRI ladder (Fermentas).

    Article Title: SMAD4 mutations causing Myhre syndrome are under positive selection in the male germline
    Article Snippet: .. Individual biological samples (sperm or blood) were processed in triplicate: each technical replicate comprising 10 μg of gDNA spiked with 0.6 ng DNA from the NsiI-Integrant cell line (corresponding to a final dilution of 3 × 10 −5 or ∼100 mutant haploid genomes) was digested in a 100 μL volume reaction containing 20 U NsiI-HF and 20 U PstI-HF (both from New England BioLabs) for 2 h at 37°C, after which another 20 U of each enzyme was added and incubated for a further 2 h. NsiI mutant sequences generate a 1,332 bp PstI fragment, while the wild-type sequences are cleaved by NsiI into 2 fragments (488 and 844 bp). .. Each digest was loaded on a 1% agarose/TAE gel and flanked by a λ DNA/EcoRI ladder (Fermentas).



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    nsii integrant cell line  (New England Biolabs)
    95
    New England Biolabs nsii integrant cell line
    Nsii Integrant Cell Line, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsii+integrant+cell+line/NsiI-HF/pmc11444041-79-25-61
    Average 95 stars, based on 1 article reviews
    nsii integrant cell line - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

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